Lipopolysaccharide Stimulates Proopiomelanocortin Gene Expression in AtT20 Corticotroph Cells

• IWASAKI Yasumasa

  Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University

• TAGUCHI Takafumi

  Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University

• NISHIYAMA Mitsuru

  Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University

• ASAI Masato

  Department of Medicine, Nagoya University Graduate School of Medicine

• YOSHIDA Masanori

  Department of Medicine, Nagoya University Graduate School of Medicine

• KAMBAYASHI Machiko

  Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University

• TAKAO Toshihiro

  Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University

• HASHIMOTO Kozo

  Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University

抄録

While lipopolysaccharides (LPS) are known to activate the hypothalamo-pituitary-adrenal axis, their direct effects on proopiomelanocortin (POMC) and adrenocorticotropin (ACTH) expression at the pituitary level through Toll-like receptors (TLRs) remain unclear. In this study, we examined the effects of LPS on ACTH secretion and the transcription of the POMC gene in the AtT20 mouse pituitary corticotroph cell line. RT-PCR analysis showed that TLR1-4 and 6 subtype mRNAs were expressed in AtT20 cells. When the cells were treated with LPS, a significant increase in the 5'-promoter activity of POMC gene was observed at 24 h, without any stimulatory effect on ACTH secretion. LPS also stimulated the expression of c-Fos gene and protein, and AP1-, but not NF-κB-, mediated transcription. Overall, our data show the expression of TLRs in the pituitary corticotroph cells, and suggest the direct stimulatory effect of LPS on POMC gene expression via TLR (probably TLR4), although the intracellular signaling pathways in the corticotroph may be different from those in immune cells.<br>

収録刊行物

• Endocrine Journal

  Endocrine Journal 55 (2), 285-290, 2008

  一般社団法人 日本内分泌学会

キーワード

• Proopiomelanocortin
• Lipopolysaccharide
• Toll-like receptor
• Innate immunity

詳細情報

• CRID: 1390282681275587840
• NII論文ID: 10021265484
• NII論文ID: 130004443390
• NII書誌ID: AA10901436
• DOI: 10.1507/endocrj.k07-075
• ISSN: 13484540
• ISSN: 09188959
• Web Site: http://www.jstage.jst.go.jp/article/endocrj/55/2/55_K07-075/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008234280
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可