Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours

• NAITO Mitsuru

  National Institute of Animal Industry

• MATSUBARA Yuko

  National Institute of Animal Industry

• HARUMI Takashi

  National Institute of Animal Industry

• TAGAMI Takahiro

  National Institute of Animal Industry

• SAKURAI Michiharu

  National Institute of Animal Health

• KUWANA Takashi

  National Institute for Minamata Disease

Abstract

Primordial germ cells isolated from the blood of early chicken embryos were transfected by lipofection for 24h and transferred to partially sterilised recipient embryos. The manipulated embryos were cultured for a further 3 days and the expression of foreign DNA (lacZ gene) in the gonads of the recipient embryos was examined. Expression of the lacZ gene was observed in the gonadal primordial germ cells of chimaeric embryos, suggesting that PGCs transfected in vitro by lipofection for 24h are still capable of migration to the germinal ridges of recipient embryos.

Journal

• Nihon Chikusan Gakkaiho

  Nihon Chikusan Gakkaiho 71 (3), 308-311, 2000

  Japanese Society of Animal Science

Keywords

• Chicken
• Primordial germ cell
• Lipofection
• lacZ gene expression
• Embryonic gonads

Details

• CRID: 1390282680173560192
• NII Article ID: 130000742604
• NII Article ID: 10021721592
• NII Book ID: AN00195188
• DOI: 10.2508/chikusan.71.308
• ISSN: 18808255
• ISSN: 00215309
• ISSN: 1346907X
• NDL BIB ID: 5349590
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5349590
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed