冷却処理後の梅山豚射出精子の運動性および先体の形態に及ぼす精漿成分の影響

• 原山 洋

  神戸大学大学院自然科学研究科

• 今野 彰

  神戸大学農学部

• 三宅 正史

  神戸大学大学院自然科学研究科

• 加藤 征史郎

  神戸大学農学部

書誌事項

タイトル別名

• Effects of Seminal Plasma Components on Motility and Acrosomal Integrity of Meishan Boar Spermatozoa after Cooling Treatments
• Effects of Seminal Plasma Components on

抄録

This study was conducted to determine effects of seminal plasma components on maintaining motility and acrosomal integrity in Meishan boar spermatozoa during cooling treatments. Ejaculates were collected from a Meishan boar by a manual method. Seminal plasma components were separated by dialysis and anion-exchange column chromatography. Spermatozoa resuspended in TCG solution containing seminal plasma or its components were cooled slowly to 4°C, or rapidly to 0°C, after which sperm motility and acrosomal integrity were examined. For those samples that were cooled slowly, the addition of seminal plasma (5-20%, v/v) exerted protective effects on maintaining sperm motility, but most spermatozoa were immobile at a high concentration (80%). However, the deleterious effects on sperm motility were not detected in seminal plasma dialyzed against TCG solution. When seminal plasma components were separated into four fractions (Fractions 1-4) by anion-exchange column chromatography, the protective effects were detected in Fractions 2 and 4. However, seminal plasma and its components hardly affected acrosomal integrity in the spermatozoa that were cooled slowly. For sperm samples that were cooled rapidly, the protective effects on acrosomal integrity as well as on sperm motility were observed in Fractions 2 and 4. These results imply that Meishan boar seminal plasma contains both beneficial and detrimental components for maintaining sperm motility during the cooling treatments. The formers are characterized by the affinity to the anion-exchange column, i.e., molecules with isoelectric points in acidic and/or neutral ranges, and the latters are passable through the dialysis tubing, i.e., low-molecular-mass molecules.

収録刊行物

• 日本畜産学会報

  日本畜産学会報 69 (8), 720-727, 1998

  公益社団法人 日本畜産学会

キーワード

• Seminal plasma
• Cooling resistance
• Spermatozoa
• Boar

詳細情報

• CRID: 1390001205196095616
• NII論文ID: 130000743089
• NII論文ID: 10021725296
• NII論文ID: 10020394521
• NII書誌ID: AN00195188
• DOI: 10.2508/chikusan.69.720
• ISSN: 18808255
• ISSN: 00215309
• ISSN: 1346907X
• NDL書誌ID: 4546359
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4546359
• Web Site: https://search.jamas.or.jp/link/ui/1999056128
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可