Genetic engineering of nitrite reductase gene improves uptake and assimilation of nitrogen dioxide by Rhaphiolepis umbellata (Thunb.) Makino

• Shigeto Jun

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• Yoshihara Sanae

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• Adam Suaad E. H.

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• Sueyoshi Kuni

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Sakamoto Atsushi

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

• Morikawa Hiromichi

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

• Takahashi Misa

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

抄録

Hypocotyl sections of Rhaphiolepis umbellata (Thunb.) Makino were transformed by Agrobacterium tumefaciens bearing a binary vector pIG121-AtNiR, which contains cDNA of the nitrite reductase gene from Arabidopsis thaliana (Atni) under the control of a modified cauliflower mosaic virus 35S promoter and chimeric hygromycin phosphotransferase gene (hph). A 4% of the hypocotyl explants transfected with Agrobacterium formed hygromycin resistant adventitious shoots, and most of them rooted upon root induction treatment. The presence and expression of the introduced transgene in putative transgenic plants (12 months after transfection) were respectively confirmed by polymerase chain reaction (PCR) analysis using primers specific to Atni and by western blot analysis using anti NiR antibody. A total of 37 transgenic plant lines were obtained. Plants 33 months after the transfection were fumigated with 200±50 ppb ¹⁵NO₂ under the natural light for one week in the fumigation chamber in a confined glasshouse. The amount of total nitrogen derived from NO₂ (reflecting uptake of NO₂) and that of Kjeldahl nitrogen derived from NO₂ (reflecting assimilation of NO₂) were determined using a mass spectrometer. One of the 9 transgenic plants tested was 1.6–2.0 times higher both in the uptake and assimilation of NO₂ than non-transformed wild-type ones.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 23 (1), 111-116, 2006

  日本植物バイオテクノロジー学会

キーワード

• nitrite reductase
• nitrogen dioxide
• phytoremediation
• <i>Rhaphiolepis umbellata</i> (Thunb.) Makino
• transgenic plant

詳細情報

• CRID: 1390001204327263488
• NII論文ID: 10021908066
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.23.111
• COI: 1:CAS:528:DC%2BD28XktFaqs7c%3D
• ISSN: 13476114
• ISSN: 13424580
• HANDLE: 10191/29871
• NDL書誌ID: 7857221
• Web Site: https://niigata-u.repo.nii.ac.jp/records/2522
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7857221
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可