Regeneration and genetic engineering of a tropical tree, Azadirachta excelsa

• Morimoto Masayuki

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Nakamura Kentaro

  Tsukuba Research Institute, Sumitomo Forestry Co., Ltd.

• Sano Hiroshi

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

Abstract

Azadirachta excelsa belongs to the family Meliaceae and is one of the most important silviculture trees in the tropics. In this study, we established a somatic embryogenesis system and succeeded in construction of transgenic lines expressing genes for Bar, GUS and GFP employing the Agrobacterium-mediated transformation technique. Somatic embryos were obtained after approximately two months of culture on Murashige-Skoog (MS) medium containing 5 μM 6-benzyladenine. Plantlets were then regenerated by transferring somatic embryos to modified 1/2 MS medium without phytohormones. We initially attempted genetic transformation using two different A. tumefaciens strains, and found only the strain LBA4404 to be active in infection to explants. The strain EHA101 was totally inactive, suggesting that a specific interaction between Agrobacterium sp. and host plant might be critical for gene transfer. The possibility is discussed of applying the methodology developed in this work to the practical propagation of tropical trees.

Journal

• Plant Biotechnology

  Plant Biotechnology 23 (1), 123-127, 2006

  Japanese Society for Plant Biotechnology

Keywords

• <i>Azadirachta excelsa</i>
• Bar
• GFP
• GUS
• somatic embryogenesis

Details

• CRID: 1390001204327264896
• NII Article ID: 10021908113
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.23.123
• COI: 1:CAS:528:DC%2BD28XktFaqsL8%3D
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 7857262
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7857262
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed