Broad range of herbicide tolerance of glutinous upland rice variety 'Yumenohatamochi' carrying human cytochrome P450 genes

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We generated three types of herbicide-tolerant Yumenohatamochi rice plants (<i>Oryza sativa</i> L cv. 'Yumenohatamochi') transformed with pIES1A1, pIJ2B6, or pIKBACH that express human cytochrome P450 gene <i>CYP1A1</i> or <i>CYP2B6</i> or co-express <i>CYP1A1</i>, <i>CYP2B6</i>, and <i>CYP2C19</i>, respectively. The transformed plants were screened by a combination of hygromycin resistance, PCR, and herbicide tolerance. We compared the tolerance towards various herbicides with different modes of action. The herbicides tested include photosynthesis inhibitors (chlorotoluron, diuron), very long-chain fatty acid (VLCFA) synthesis inhibitors (acetochlor, alachlor, metolachlor, and mefenacet), a carotenoid biosynthesis inhibitor (norflurazon), and microtubule assembly inhibitors (pendimethalin, trifluralin, and amiprophos-methyl). The pIES1A1- or pIJ2B6-transformed Yumenohatamochi showed tolerance towards five and four different chemical classes of herbicides, respectively. The spectrum of herbicide tolerance of pIKBACH-transformed Yumenohatamochi was broader compared to transformants expressing the single human P450 gene. Transformed rice plants expressing P450s involved in xenobiotic metabolism may become useful tools for the breeding of herbicide-tolerant crops.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 23(2), 227-231, 2006-03-01

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021908572
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    7857370
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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