In vitro plant regeneration from the petioles of primary leaves of mungbean Vigna radiata L.

• Sita Mahalakshmi L.

  Department of Botany, Osmania University

• Leela T.

  Department of Botany, Osmania University

• Kiran Kumar B.

  Department of Botany, Osmania University

• Naresh B.

  Department of Botany, Osmania University

• Devi Prathibha

  Department of Botany, Osmania University

抄録

High frequency in vitro plant regeneration via multiple shoots is reported from petioles of leaf explants of mungbean (Vigna radiata L.) on B5 culture medium supplemented with 6-benzyladenine (BA). The explants comprised of petiole cut at the node bearing the basal half of the lamina. Regeneration frequency varied with age of explant source and concentration of BA. Explants produced shoot-buds and shoots within 2 weeks. The explants harvested from 10-day-old seedlings produced higher number of shoots (8–9) than those from 4-day-old seedlings. Explants consisting of the petiole bearing either the entire lamina or atleast the basal half were more responsive than the explants without lamina. Histological studies confirmed the adventitious nature of shoot-buds. Shoots were rooted on B5 medium supplemented with 0.5 mg 1⁻¹ indolebutyric acid. The plantlets, hardened and transferred to soil with 90% survival developed normal flowers and viable seeds. This efficient plant regeneration protocol can be used for genetic transformation to produce transgenic mungbean.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 23 (4), 409-411, 2006

  日本植物バイオテクノロジー学会

キーワード

• <i>In vitro</i> plant regeneration
• <i>Vigna radiata</i>
• petiole
• primary leaves

詳細情報

• CRID: 1390001204329098624
• NII論文ID: 10021909229
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.23.409
• COI: 1:CAS:528:DC%2BD28XhtFSjsr7F
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 8041148
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8041148
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可