Efficient plant regeneration from protoplasts of eggplant rootstock cultivar and its wild relatives

• Iwamoto Yuzuri

  Agricultural, Food and Environmental Sciences, Research Center of Osaka Prefecture

• Ezura Hiroshi

  Gene Research Center, Graduate School of Life and Environmetal Sciences, University of Tsukuba

抄録

Protoplast culture and shoot regeneration from protoplast-derived calli were compared among different organs of Solanum integrifolium and three of its wild relatives (S. abutiloides, S. scabrum, and S. toxicarium). Leaves, cotyledons, and hypocotyls were used as sources of protoplast preparation. After one month of culture, a high frequency of visible colony formation was obtained from cotyledon protoplasts of S. integrifolium and S. scabrum, hypocotyl protoplasts of S. integrifolium and S. abutiloides, and leaf protoplasts of S. integrifolium and S. toxicarium. In addition, when the primary culture was started at a density of 2.5−5×10⁴ protoplasts ml⁻¹, the highest frequency of colony formation was obtained. Moreover, when the colonies were subcultured for 7 days on solid callus-proliferation medium before being transferred to shoot-induction medium, the plant regeneration frequency improved to between 91.8 and 98.8%.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 23 (5), 525-529, 2006

  日本植物バイオテクノロジー学会

キーワード

• eggplant rootstock
• plant regeneration
• protoplast culture
• wild relatives

詳細情報

• CRID: 1390282679304057216
• NII論文ID: 10021909694
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.23.525
• ISSN: 13476114
• ISSN: 13424580
• HANDLE: 10191/17588
• NDL書誌ID: 8571227
• Web Site: https://niigata-u.repo.nii.ac.jp/records/2521
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8571227
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可