Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser
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- Kajiyama Shin'ichiro
- Department of Biotechnology, Faculty of Engineering, Osaka University
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- Inoue Fumihide
- Department of Biotechnology, Faculty of Engineering, Osaka University
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- Yoshikawa Yoshinobu
- Department of Biotechnology, Faculty of Engineering, Osaka University
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- Shoji Takeshi
- Department of Biotechnology, Faculty of Engineering, Osaka University
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- Fukusaki Eiichiro
- Department of Biotechnology, Faculty of Engineering, Osaka University
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- Kobayashi Akio
- Department of Biotechnology, Faculty of Engineering, Osaka University
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Abstract
Novel methods for foreign-gene introduction into specific cells are required for the functional analysis of specific cells. We report a new transfection method for intact plant cells that uses ArF excimer laser-induced shock waves to introduce foreign gene-coated gold particles into plant cells through the cell wall. Foreign gene (sGFP)-coated gold particles were randomly scattered on the surface of Torenia tissue samples; the laser was irradiated toward the gold particles using a hollow optical fiber and a quartz light-condensing tip to introduce the particles into the target cells. Treated plant tissue samples were then cultured for several weeks on the selection medium, and transformation was assessed by fluorescence microscopy and PCR. Irradiation-induced damage to the cells and coated DNA was insignificant, and the transformation efficiency was similar to that of biolistic transformation based on the particle number. Although this method requires a suitable laser setup and is time-consuming, it could be applied in transformation experiments on specific target cells. Moreover, this method can possibly be used to introduce not only DNA but also biologically active substances such as protein regulators or inhibitors, whose introduction into cells is difficult through the cell wall.
Journal
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- Plant Biotechnology
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Plant Biotechnology 24 (3), 315-320, 2007
Japanese Society for Plant Biotechnology
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Details 詳細情報について
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- CRID
- 1390282679305053440
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- NII Article ID
- 10021911546
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD2sXosVejsL8%3D
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- ISSN
- 13476114
- 13424580
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- NDL BIB ID
- 8775231
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed