Agrobacterium-mediated transformation of indica rice under Acetosyringone-free conditions

この論文にアクセスする

この論文をさがす

著者

抄録

The possibility of developing transgenic <i>indica</i> rices through <i>Agrobacterium</i>-mediated transformation in the absence of acetosyringone at bacterial preinduction or co-cultivation or both stages was assessed. Four-week-old, scutellum derived calluses of <i>indica</i> rice (<i>Oryza sativa</i> L. cv. ‘Pusa Basmati1’) were co-cultivated with <i>A. tumefaciens</i> strain LBA4404 (pSB1), harboring the binary vector pCAMBIA 1301 with the β-glucuronidase (GUS) and hygromycin phosphotransferase (HPT) genes in the T-DNA region. Addition of acetosyringone (AS) to both preinduction medium (PIM) and co-cultivation medium (CCM) induced higher levels of transient GUS expression than that obtained with the addition of AS to either of the stages. Addition of only sucrose to both preinduction and co-cultivation media yielded transient expression levels similar to those obtained by the addition of AS. The resultant fertile plants were stable transformants as revealed by GUS histochemical assay and PCR analysis for the GUS and HPT genes. Thus, phenolics like AS may not be essential for induction of <i>vir</i> genes, and development of transgenic <i>indica</i> rice is feasible under AS-free conditions.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 24(5), 507-511, 2007-12-01

    日本植物細胞分子生物学会

参考文献:  21件中 1-21件 を表示

各種コード

  • NII論文ID(NAID)
    10021912326
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    9302528
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ