Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene

• Aida Ryutaro

  National Institute of Floricultural Science, National Agriculture and Food Research Organization

• Narumi Takako

  National Institute of Floricultural Science, National Agriculture and Food Research Organization

• Ohtsubo Norihiro

  National Institute of Floricultural Science, National Agriculture and Food Research Organization

• Yamaguchi Hiroyasu

  National Institute of Floricultural Science, National Agriculture and Food Research Organization

• Kato Ko

  Nara Institute of Science and Technology

• Shinmyo Atsuhiko

  Nara Institute of Science and Technology

• Shibata Michio

  National Institute of Floricultural Science, National Agriculture and Food Research Organization

抄録

We investigated the efficiency of the 5′-untranslated region (UTR) of the tobacco alcohol dehydrogenase gene (NtADH-5′UTR) as a translational enhancer in chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri). Three constructs were introduced: control, “ADH−,” cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::β-glucuronidase (GUS); NtADH-5′UTR with a spacer, “ADH+S,” CaMV 35S promoter::NtADH-5′UTR::25-bp spacer::GUS; and NtADH-5′UTR with no spacer, “ADH+,” CaMV 35S promoter::NtADH-5′UTR::GUS. The highest GUS activity in ADH+S and ADH+ for chrysanthemum was about 45 and 190 times, respectively, and for torenia was 12 and 22 times, respectively, than that for the ADH− plants. NtADH-5′UTR enhanced translational efficiency in both species. With the lowest translational efficiency set to 1, the relative translational efficiencies were 1 to 15 (ADH−), 47 to 568 (ADH+S), and 267 to 1360 (ADH+) for chrysanthemum, and 1 to 3 (ADH−), 47 to 114 (ADH+S), and 85 to 226 (ADH+) for torenia. NtADH-5′UTR would facilitate practical breeding and fundamental genetic research in chrysanthemum and torenia.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 25 (1), 69-75, 2008

  日本植物バイオテクノロジー学会

キーワード

• <i>Chrysanthemum morifolium</i>
• molecular breeding
• ornamental plants
• <i>Torenia fournieri</i>
• translational enhancer

詳細情報

• CRID: 1390001204327925248
• NII論文ID: 10021912665
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.25.69
• ISSN: 13476114
• ISSN: 13424580
• ISSN: http://id.crossref.org/issn/13424580
• NDL書誌ID: 9423484
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9423484
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可