LET-dependent effects of heavy-ion beam irradiation in Arabidopsis thaliana
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- Kazama Yusuke
- RIKEN Nishina Center
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- Saito Hiroyuki
- RIKEN Nishina Center
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- Yamamoto Yoshiharu Y.
- Center for Gene Research, Nagoya University
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- Hayashi Yoriko
- RIKEN Nishina Center
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- Ichida Hiroyuki
- RIKEN Nishina Center
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- Ryuto Hiromichi
- RIKEN Nishina Center
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- Fukunishi Nobuhisa
- RIKEN Nishina Center
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- Abe Tomoko
- RIKEN Nishina Center
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Abstract
We irradiated Arabidopsis thaliana with several kinds of heavy-ion beams to investigate the linear energy transfer (LET)-dependent effects of heavy-ion beam irradiation. First, dry seeds were irradiated with C, N, Ne, Ar, or Fe ions at doses ranging from 5 to 400 Gy to compare the flowering and mutation rates among the ion species. The sensitivity of the flowering and mutation rates to irradiation differed markedly among the ion species. Of the ion species, N (30 keV μm−1) was the most effective at inducing albino plants. Second, we examined the effects of LET on mutation induction. The LET of C, N, Ne, and Ar ion beams was controlled at 30–640 keV μm−1. Regardless of ion species, irradiation with the same LET value resulted in the same flowering rate and mutation rate. Thus, the LET of ion beams seems to be an important factor affecting mutagenesis. We found a 440-bp deletion in the hy (elongated hypocotyl) mutant that was isolated from M2 progeny. These fundamental data on LET-dependence can be used to develop advanced technologies for plant mutagenesis.
Journal
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- Plant Biotechnology
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Plant Biotechnology 25 (1), 113-117, 2008
Japanese Society for Plant Biotechnology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679304621952
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- NII Article ID
- 10021912820
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- NII Book ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD1cXkslKmsbg%3D
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- ISSN
- 13476114
- 13424580
- http://id.crossref.org/issn/13424580
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- NDL BIB ID
- 9423574
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed