Characterization of PAP1-upregulated Glutathione S-transferase genes in Arabidopsis thaliana

• Wangwattana Bunyapa

  Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University

• Koyama Yoko

  Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University

• Nishiyama Yasutaka

  Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University Institute of Life Science, Ehime Women's College

• Kitayama Masahiko

  Institute of Life Science, Ehime Women's College

• Yamazaki Mami

  Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University CREST, Japan Science and Technology Agency

• Saito Kazuki

  Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University RIKEN Plant Science Center

Bibliographic Information

Other Title

• Gene note: Characterization of PAP1-upregulated glutathione S-transferase genes in Arabidopsis thaliana

Abstract

Glutathione S-transferase (GST) plays an important role in the transport and accumulation of anthocyanin and proanthocyanidin in plants. In our previous study on Arabidopsis thaliana overexpressing the PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) gene encoding an MYB transcription factor, the AtGSTF5 and AtGSTF6 genes encoding GST-like protein were up-regulated along with TRANSPARENT TESTA 19 (TT19), which is required for the accumulation of anthocyanin and proanthocyanidin. The proteins encoded by these 3 genes showed very weak GST activities as detected by using recombinant proteins expressed in Escherichia coli. The anthocyanin levels were severely decreased in the tt19 mutant but not in the Atgstf6 mutant, suggesting that TT19 is almost exclusively involved in anthocyanin accumulation. The results of co-expression network analysis using public transcriptome data corresponded to the proposition of the predominant role of TT19 in anthocyanin accumulation.

Journal

• Plant Biotechnology

  Plant Biotechnology 25 (2), 191-196, 2008

  Japanese Society for Plant Biotechnology

Keywords

• anthocyanin
• <i>Arabidopsis thaliana</i>
• glutathione <i>S</i>-transferase
• <i>TT19.</i>

Details

• CRID: 1390282679304409088
• NII Article ID: 10021913113
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.25.191
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 9423002
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9423002
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed