Isolation and characterization of a cDNA coding cowpea (Vigna unguiculata (L.) Walp.) calcineurin B-like protein-interacting protein kinase, VuCIPK1
-
- Imamura Masakazu
- Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
-
- Yuasa Takashi
- Department of Plant Resources, Faculty of Agriculture, Kyushu University
-
- Takahashi Tomoko
- Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
-
- Nakamura Nagisa
- Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
-
- Htwe Nang Myin Phyu Sin
- Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
-
- Zheng Shao-Hui
- Coastal Bioenvironment Center, Saga University
-
- Shimazaki Ken-ichiro
- Department of Biology, Graduate School of Sciences, Kyushu University
-
- Iwaya-Inoue Mari
- Department of Plant Resources, Faculty of Agriculture, Kyushu University
Search this article
Abstract
We isolated a calcineurin B-like protein (CBL)-interacting protein kinase (EC 2.7.11.1) homolog (VuCIPK1) from cowpea (Vigna unguiculata (L.) Walp.) with significant similarity to AtCIPK3 (86% amino acid identity), which is involved in K+ transport. VuCIPK1 mRNA was detected in the whole plant by RT-PCR. Any significant change in VuCIPK1 mRNA levels was not detected when cowpea was subjected to various environmental stresses. Immunoblot assay with an anti-VuCIPK1 specific antibody and an anti-CBL antibody showed that immunologically CIPK- and CBL-related polypeptides were preferentially associated with membrane fractions of cowpea. The anti-VuCIPK1 antibody cross-reacted to two different CIPK-related polypeptides with relative molecular masses of 51 kDa in root and 55 kDa in mature leaf. Immunoprecipitation assay by anti-VuCIPK1 antibody using cowpea leaf extracts showed that endogenous VuCIPK1 was autophosphorylated on the threonine residues in response to salt stress. The recombinant VuCIPK1 phosphorylated casein in the presence of Mn2+. These observations suggest that membrane-associated VuCIPK1 is involved osmotic stress-activated kinase regulated by its phosphorylation status.
Journal
-
- Plant Biotechnology
-
Plant Biotechnology 25 (5), 437-445, 2008
Japanese Society for Plant Biotechnology
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001204328473344
-
- NII Article ID
- 10021914551
-
- NII Book ID
- AA11250821
-
- ISSN
- 13476114
- 13424580
-
- NDL BIB ID
- 9654473
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed