Infrequent-Restriction-Site PCR(IRS-PCR)法による大腸菌O157分離株のDNAタイピング

• 村瀬 敏之

  神奈川県衛生研究所・細菌病理部

• 大橋 美幸

  麻布大学・環境保健学部

• 山井 志朗

  神奈川県衛生研究所・細菌病理部

• 丸山 務

  麻布大学・環境保健学部

書誌事項

タイトル別名

• DNA Fingerprinting of Escherichia coli O157 by Infrequent-Restriction-Site PCR.
• Ｉｎｆｒｅｑｕｅｎｔ‐Ｒｅｓｔｒｉｃｔｉｏｎ‐Ｓｉｔｅ　ＰＣＲ（ＩＲＳ‐ＰＣＲ）法による大腸菌Ｏ１５７分離株のＤＮＡタイピング
• Infrequent Restriction Site PCR IRS PCR ホウ ニ ヨル ダイチョウキン O157 ブンリカブ ノ DNA タイピング

抄録

Pulsed-field gel electrophoresis (PFGE) has been used as a reliable epidemiological tool to identify the possible source and route of infection by Shiga-toxin producing Escherichia coli (STEC) O157. However, it has been found that some strains could not be subjected to the analysis because their DNAs are easily degraded. To produce strain-specific profiles of such strains, we used a method of amplifying DNA sequences flanking infrequent restriction sites (infrequent-restriction-site PCR; IRS-PCR) . Fifty-two strains of E. coli O157 were typed into 12 and 14 types by IRS-PCR patterns from DNAs digested with XbaI and HhaI and those with BlnI and HhaI, respectively. These data suggest that selection of an appropriate set of restriction enzymes will provides more discrimination patterns. In 18 of the 52 strains, that gave individual PFGE patterns, 11 IRS-PCR patterns were shown. Remaining 34 strains that were untypeable by PFGE were classified into 3 IRS-PCR types, indicating that some of those strains were possibly related.

収録刊行物

• 獣医疫学雑誌

  獣医疫学雑誌 4 (2), 59-66, 2000

  獣医疫学会

詳細情報

• CRID: 1390001204338248192
• NII論文ID: 10022000652
• NII書誌ID: AA11157349
• DOI: 10.2743/jve.4.59
• ISSN: 18812562
• ISSN: 13432583
• NDL書誌ID: 5657588
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030621543
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5657588
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可