Effects of Increased Intracellular Cl〔-〕 Concentration on Membrane Responses to Acetylcholine in the Isolated Endothelium of Guinea Pig Mesenteric Arteries
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- Yamamoto Yoshimichi
- Laboratory of Physiology, Nagoya City University School of Nursing Department of Regulatory Cell Physiology, Nagoya City University Graduate School of Medical Sciences
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- Suzuki Hikaru
- Department of Regulatory Cell Physiology, Nagoya City University Graduate School of Medical Sciences
書誌事項
- タイトル別名
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- Effects of Increased Intracellular Cl- Concentration on Membrane Responses to Acetylcholine in the Isolated Endothelium of Guinea Pig Mesenteric Arteries
- Effects of Increased Intracellular Cl− Concentration on Membrane Responses to Acetylcholine in the Isolated Endothelium of Guinea Pig Mesenteric Arteries
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ACh-induced membrane responses in vascular endothelial cells that have been reported vary between preparations from a sustained hyperpolarization to a transient hyperpolarization followed by a depolarization; the reason for this variation is unknown. Using the perforated whole-cell clamp technique, we investigated ACh-induced membrane currents in freshly isolated endothelial layers having a resting membrane potential of less negative than −10 mV. A group of cells was electrically isolated using a wide-bore micropipette, and their membrane potential was well controlled. ACh activated K+ and Cl− currents simultaneously. The K+ current was blocked by a combination of charybdotoxin and apamin and appears to result from the opening of IKCa and SKCa channels. The Cl− current was partially blocked by tamoxifen, niflumic acid, or DIDS and appears to be produced by Ca2+-activated Cl− channels. When the pipettes contained 20 mM Cl−, the ACh-induced K+ conductance started decreasing during a 1-min application of ACh while the Cl− conductance continued, making the ACh-induced hyperpolarization sustained. When the pipettes contained 150 mM Cl−, both conductances started decreasing during a 1-min application of ACh, making the ACh-induced hyperpolarization small and transient. [Cl−]i is very likely modified by experimental procedures such as the cell isolation and the intracellular dialysis with the pipette solution. Such a variability in [Cl−]i may be one of the reasons for the variations in the ACh-induced membrane response.<br>
収録刊行物
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- The Journal of Physiological Sciences
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The Journal of Physiological Sciences 57 (1), 31-41, 2007
一般社団法人 日本生理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680228873600
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- NII論文ID
- 10022596056
- 130004466877
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- NII書誌ID
- AA12129145
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- ISSN
- 18806562
- 18806546
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- NDL書誌ID
- 8662024
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可