Direct Comparison between ICSI-Mediated DNA Transfer and Pronuclear DNA Microinjection for Producing Transgenic Rats

• HIRABAYASHI Masumi

  National Institute for Physiological Sciences The Graduate University for Advanced Studies

• KATO Megumi

  National Institute for Physiological Sciences

• AMEMIYA Kazue

  National Institute for Physiological Sciences

• HOCHI Shinichi

  Faculty of Textile Science and Technology, Shinshu University

抄録

Production efficiency of transgenic rats was compared directly between the routine pronuclear microinjection of exogenous DNA solution (PNMI-Tg method) and the ooplasmic injection of sperm cells exposed to exogenous DNA solution (ICSI-Tg method) using six DNA constructs. The overall production efficiency per treated oocyte in the ICSI-Tg method (mean 1.1%, range 0.2 to 3.1%) was similar to that in the PNMI-Tg method (mean 1.1%, range 0 to 2.4%). An advantage of the ICSI-Tg method in the production of transgenic rats is noted in cases in which a low yield of pronuclear zygotes is an inevitable fate of the rat strain.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 57 (2), 145-148, 2008

  公益社団法人 日本実験動物学会

キーワード

• ICSI
• pronuclear injection
• transgenic rats

詳細情報

• CRID: 1390282680024226816
• NII論文ID: 130000067993
• NII論文ID: 10024166309
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.57.145
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 9455656
• Web Site: http://id.ndl.go.jp/bib/9455656
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9455656
• Web Site: http://www.jstage.jst.go.jp/article/expanim/57/2/57_2_145/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008232150
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可