An Efficient Reproductive Method for Irs2-/- Mice with C57BL/6JJcl Genetic Background

• HASHIMOTO Haruo

  Central Institute for Experimental Animals

• ETO Tomoo

  Central Institute for Experimental Animals

• KAMISAKO Tsutomu

  Central Institute for Experimental Animals

• HOYA Naoko

  Central Institute for Experimental Animals

• HATAKEYAMA Teruhiko

  Central Institute for Experimental Animals

• ARAI Toshiro

  Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, Nippon Veterinary and Animal Science University

• YOKOSUKA Makoto

  Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, Nippon Veterinary and Animal Science University

• OHNISHI Yasuyuki

  Central Institute for Experimental Animals

• ITO Mamoru

  Central Institute for Experimental Animals

• HIOKI Kyoji

  Central Institute for Experimental Animals

• SUZUKI Ryo

  Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo

• OHSUGI Mitsuru

  Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo

• SAITO Muneo

  Central Institute for Experimental Animals

• UEYAMA Yoshito

  Central Institute for Experimental Animals Department of Pathology, Tokai University School of Medicine

• YAMAUCHI Toshimasa

  Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo

• KUBOTA Naoto

  Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo

• TOBE Kazuyuki

  Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo

• KADOWAKI Takashi

  Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo

• TAMAOKI Norikazu

  Central Institute for Experimental Animals

• NOMURA Tatsuji

  Central Institute for Experimental Animals

• KOSAKA Kinori

  Central Institute for Experimental Animals Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo

Abstract

Efficient reproduction using natural mating and reproduction technology [in vitro fertilization (IVF) and embryo transfer (ET)] was investigated in IRS2 deficient mice with C57BL/6JJcl genetic background (Irs2^-/- mice) as a typical type 2 diabetes model. From the results using various combinations of Irs2^-/- and Irs2^-/+ mice, the combination of female Irs2^-/+ × male Irs2^-/- was found to be more efficient than other combinations. In applications of reproduction technology using IVF and ET, the combination of female Irs2^-/+ × male Irs2^-/- involves the possibility of Irs2^-/- production by repeats using female Irs2^-/+ mice. However, reproductive continuity using this combination is difficult because of dependence on human technique and the cost of ET. Therefore, we concluded that Irs2^-/- mice should be produced by embryo transfer using Irs2^-/- mice from a colony consisting of female Irs2^-/+ × male Irs2^-/-.<br>

Journal

• Experimental Animals

  Experimental Animals 57 (4), 407-411, 2008

  Japanese Association for Laboratory Animal Science

Keywords

• diabetes mellitus
• <i>Irs2^-/-</i> mice
• reproduction

Details

• CRID: 1390001205044066048
• NII Article ID: 130000067982
• NII Article ID: 10024166620
• NII Book ID: AA11032321
• DOI: 10.1538/expanim.57.407
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL BIB ID: 9566144
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9566144
• Web Site: http://www.jstage.jst.go.jp/article/expanim/57/4/57_4_407/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009001467
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed