Significance of Molecular Diagnosis using Histopathological Specimens in Cestode Zoonoses
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- Yamasaki Hiroshi
- Department of Parasitology, Asahikawa Medical College Department of Parasitology, National Institute of Infectious Diseases
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- Nakaya Kazuhiro
- Animal Laboratory for Medical Research, Asahikawa Medical College
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- Nakao Minoru
- Department of Parasitology, Asahikawa Medical College
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- Sako Yasuhito
- Department of Parasitology, Asahikawa Medical College
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- Ito Akira
- Department of Parasitology, Asahikawa Medical College
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Abstract
Cestode zoonosis cases confirmed by PCR-based mitochondrial DNA analysis were investigated. The cestodiosis included taeniasis, cysticercosis, alveolar echinococcosis, cystic echinococcosis, sparganosis mansoni, diphyllobothriasis and diplogonoporiasis. DNA samples were extracted from the ethanol-fixed, formalin-fixed, paraffin-embedded sections, HE-stained, and the PAS- or acetocarmine-stained samples submitted for histopathology. For PCR-based analysis, cytochrome c oxidase subunit 1 and⁄or cytochrome b genes were amplified by multiplex PCR or conventional PCR coupled with DNA sequencing. Although DNA molecules were degraded in most formalin-fixed samples, smaller gene fragments were successfully amplified and the species causing cestodiosis could be identified by DNA sequence analysis of the amplicons. This review describes cestode zoonosis cases in which mitochondrial DNA analysis was useful not only for routine and retrospective diagnosis, but also for genetic polymorphism analysis and molecular identification of the species associated with pathogenicity. The significance of molecular diagnosis using histopathological specimens for cestode zoonoses is also discussed.
Journal
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- Tropical Medicine and Health
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Tropical Medicine and Health 35 (4), 307-321, 2007
Japanese Society of Tropical Medicine
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Details 詳細情報について
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- CRID
- 1390001205218920704
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- NII Article ID
- 10024174580
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- NII Book ID
- AA11912846
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- ISSN
- 13494147
- 13488945
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed