表面赤外分光法を用いた抗原抗体反応の非標識検出：二次構造解析による特異・非特異信号の識別

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タイトル別名
  • Surface Infrared Spectroscopic Study on Label-Free Detection of Antigen-Antibody Interactions: Discrimination between Specific and Nonspecific Signals using Protein Secondary Structure Analysis
  • ヒョウメン セキガイ ブンコウホウ オ モチイタ コウゲン コウタイ ハンノウ ノ ヒヒョウシキ ケンシュツ 2ジ コウゾウ カイセキ ニ ヨル トクイ ヒトクイ シンゴウ ノ シキベツ

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This paper reviews our recent approaches for a label-free detection of protein-protein interactions which can perform simultaneous protein conformational analysis by using infrared absorption spectroscopy in the multiple internal reflection geometry (MIR-IRAS). Using this method, the target protein was detected in aqueous solution phase based on the peak height of the protein amide I and amide II bands, while discrimination of specific and nonspecific signals is made based on the secondary structure of the target protein. An antigen-antibody interaction on semiconductor surfaces was investigated as a model system, since it is well-known that antibodies largely consist of β-sheet structures. The features of our approach using MIR-IRAS combined with protein secondary structure analysis were discussed in terms of sensitivity, capability of quantitative analysis, and specific/nonspecific discrimination.

収録刊行物

  • 表面科学

    表面科学 29 (9), 558-563, 2008

    公益社団法人 日本表面科学会

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