Molecular Cloning of a Porcine (Sus scrofa) Apoptosis Inhibitory Ligand, Netrin-1, and Its Receptor, p53RDL1

• MAEDA Akihisa

  Animal Resource Science Center, The University of Tokyo

• MATSUDA Fuko

  Animal Resource Science Center, The University of Tokyo

• GOTO Yasufumi

  Animal Resource Science Center, The University of Tokyo

• CHENG Yuan

  Animal Resource Science Center, The University of Tokyo

• GONDA Hiroshi

  Animal Resource Science Center, The University of Tokyo

• INOUE Naoko

  Laboratory of Animal Morphology and Function, Graduate School of Bioagricultural Sciences, Nagoya University

• NAKAGAWA Shoma

  Animal Resource Science Center, The University of Tokyo

• MANABE Noboru

  Animal Resource Science Center, The University of Tokyo Research Center for Food Safety, The University of Tokyo

抄録

The apoptosis inhibitory ligand (Netrin-1) and its receptor (p53-regulated receptor for death and life: p53RDL1) play an important role in the regulation of selective apoptosis. When Netrin-1 binds to p53RDL1, p53-dependent apoptosis is inhibited. We identified porcine (Sus scrofa) cDNAs encoding Netrin-1 [pNetrin-1; 1,803 base pairs (bp) and 600 amino acids (aa)] and p53RDL1 (pp53RDL1; 2,838 bp and 945 aa). Porcine p53RDL1 (pp53RDL1) contains a death domain (DD), a tandem specific amino acid region, in its C-terminal, suggesting that it mediates death signaling by binding with other pro-apoptotic factors via the DD. Porcine Netrin-1 (pNetrin-1), pp53RDL1 and the DD in pp53RDL1 showed high levels of identity in aa sequence with human and murine Netrin-1 (98 and 97%, respectively), p53RDL1 (94 and 91%, respectively) and the DD in p53RDL1 (96 and 95%, respectively). Reverse transcription-polymerase chain reaction (RT-PCR) revealed that the levels of pNetrin-1 and pp53RDL1 mRNAs were moderate in granulosa cells compared with their expression in other tissues and that their levels during follicular atresia were stable. The Netrin-1 and p53RDL1 system may regulate the induction of apoptosis in porcine granulosa cells.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 54 (4), 275-280, 2008

  公益社団法人 日本繁殖生物学会

キーワード

• Apoptosis inhibitory ligand (Netrin-1)
• Granulosa cell
• Porcine (<i>Sus scrofa</i>)
• p53-Regulated receptor for death and life (p53RDL1)

詳細情報

• CRID: 1390001206337025920
• NII論文ID: 10024289440
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.20044
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 9621680
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9621680
• Web Site: http://www.jstage.jst.go.jp/article/jrd/54/4/54_20044/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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