Muscarinic Receptor Subtypes Mediating Positive and Negative Inotropy in the Developing Chick Ventricle

• Nouchi Hideaki

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

• Kaeriyama Seri

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

• Muramatsu Akiko

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

• Sato Masumi

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

• Hirose Kunihiko

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

• Shimizu Nagako

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

• Tanaka Hikaru

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

• Shigenobu Koki

  Department of Pharmacology, Toho University School of Pharmaceutical Sciences, Japan

抄録

The inotropic response to muscarinic receptor stimulation of isolated chick ventricular myocardium was examined at various developmental stages, and the receptor subtype involved was pharmacologically characterized. In embryonic chick ventricles, carbachol (CCh) produced positive inotropy at micromolar concentrations. In hatched chick ventricles, CCh produced negative inotropy at nanomolar concentrations. Neither positive nor negative inotropy was observed in the 19 – 21-day-old embryos. Both positive and negative inotropy were also observed with acetylcholine and oxotremoline-M. The CCh-induced positive inotropy in 7 – 9-day-old embryonic ventricles and the negative inotropy in 1 – 3-day-old hatched chick ventricles were antagonized by muscarinic receptor antagonists; pA₂ values for the positive and negative responses of pirenzepine were 7.5 and 7.2, those of AF-DX116 (11-[(2-[(diethylamino)methyl]-1-piperidinyl)acetyl]-5,11-dihydro-6H-pyrido[2,3-b][1,4] benzodiazepine-6-one) were 6.8 and 6.9, those of 4-diphenylacetoxy-N-methylpiperidine (4-DAMP) were 9.0 and 8.5, and those of himbacine were 7.0 and 8.0, respectively. CCh had no effect on action potential configuration. In conclusion, the positive inotropy is most likely mediated by muscarinic M₁ receptors and the negative inotropy is mostly likely mediated by muscarinic M₄ receptors.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 103 (1), 75-82, 2007

  公益社団法人 日本薬理学会

キーワード

• carbachol
• chick
• ventricle
• muscarinic receptor
• contractile force

詳細情報

• CRID: 1390282680153599616
• NII論文ID: 10024312068
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.fpj06013x
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 8617981
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8617981
• Web Site: https://search.jamas.or.jp/link/ui/2007192158
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可