N,N-dimethyl-D-erythro-sphingosine inhibits store-operated Ca[2+] entry in U937 monocytes
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- Jo Ji-Yeong
- Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Life Science and Technology Institutes, Pusan National University, Korea
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- Kim Hyo-Lim
- Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Life Science and Technology Institutes, Pusan National University, Korea
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- Lee Yun-Kyung
- Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Life Science and Technology Institutes, Pusan National University, Korea
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- Tomura Hideaki
- Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Japan
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- Bae Yoe-Sik
- Department of Biochemistry, College of Medicine, Dong-A University, Korea
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- Okajima Fumikazu
- Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Japan
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- Im Dong-Soon
- Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Life Science and Technology Institutes, Pusan National University, Korea
書誌事項
- タイトル別名
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- N,N-Dimethyl-<sc>D</sc>-erythro-Sphingosine Inhibits Store-Operated Ca2+ Entry in U937 Monocytes
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Calcium is a ubiquitous second messenger that controls a broad range of cellular functions, and store-operated calcium entry (SOCE) is the primary mechanism of regulated Ca2+ entry in non-excitable immunocytes. In this study, we found that N,N-dimethyl-<sc>D</sc>-erythro-sphingosine (DMS) inhibited SOCE. In U937 cells, treatment with DMS for 2 h inhibited thapsigargin-induced SOCE by about 70%. DMS inhibited SOCE in a concentration-dependent manner when it was added to the cells after SOCE reached a plateau. DMS-induced SOCE inhibition was also confirmed by the Mn2+-quenching method, which monitors only Ca2+ influx. Because sphingosine kinase inhibitors or protein kinase C (PKC) inhibitors could not mimic the SOCE inhibition, sphingosine kinase and PKC could be excluded as targets of DMS-induced inhibition of SOCE. Furthermore, disruption of lipid rafts with methyl-β-cyclodextrin and bacterial sphingomyelinase did not influence DMS-induced inhibition of SOCE. DMS-induced inhibition of SOCE in U937 human monocytes is a unique observation and could serve as a basis to study modulation of intracellular Ca2+ concentration by sphingolipids, although the precise mechanism should be elucidated in the future.<br>
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 107 (3), 303-307, 2008
公益社団法人 日本薬理学会
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詳細情報
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- CRID
- 1390001205179743488
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- NII論文ID
- 10024321429
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- NII書誌ID
- AA11806667
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 9583181
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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