Measurement of chloride concentrations of insensible sweat collected from thumb

• NAKAKUKI Miyuki

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

• ISHIGURO Hiroshi

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

• SHIROTA Keiichi

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

• YAMAMOTO Akiko

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

• KO Shigeru B. H.

  Department of Gastroenterology, Nagoya University Graduate School of Medicine

• GOTO Hidemi

  Department of Gastroenterology, Nagoya University Graduate School of Medicine

• FUJIKI Kotoyo

  Department of Nutritional Sciences, Nagoya University of Arts and Sciences

• KONDO Takaharu

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

• ENDO Akira

  Department of Pediatrics, Hamamatsu Medical Center

• NARUSE Satoru

  Miyoshi Municipal Hospital

Bibliographic Information

Other Title

• 汗中クロライド濃度の簡便な測定法の開発

Abstract

To evaluate the dysfunction of cystic fibrosis transmembrane conductance regulator (CFTR) Cl^- channel in patients with cystic fibrosis (CF) and chronic pancreatitis, we established a method to measure the sweat Cl^- concentrations ([Cl^-]) of insensible sweat from the thumb. The sweat rate from one thumb was measured by a perspiration meter and sweat during the period was collected from the other thumb by holding a small sampling tube containing 100 μl water. The Cl^- content was measured either by capillary electrophoresis or by a highly sensitive Cl^--selective electrode, both of which were sensitive enough to measure 0.06-1.2mM Cl^- in the sample. The sweat [Cl^-] in healthy Japanese was 38.0±20.9mM (mean±SD, n=73) and was correlated with the age (p<0.01). The sweat [Cl^-] in a 2-year-old CF patient was 156mM. Measurement of sweat [Cl^-] from the thumb using a Cl^--selective electrode is a simple and reliable tool to evaluate the CFTR function.<br>

Journal

• Suizo

  Suizo 23 (4), 486-493, 2008

  Japan Pancreas Society

Keywords

• Sweat chloride concentration
• Cystic fibrosis
• Chronic pancreatitis
• Insensible sweat
• CFTR

Details

• CRID: 1390001204638709632
• NII Article ID: 10024367756
• NII Article ID: 130004495812
• NII Book ID: AN10043798
• DOI: 10.2958/suizo.23.486
• ISSN: 18812805
• ISSN: 09130071
• Web Site: https://search.jamas.or.jp/link/ui/2008319839
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed