Knockout of glutelin genes which form a tandem array with a high level of homology in rice by gamma irradiation
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- Morita Ryouhei
- Institute of Radiation Breeding, National Institute of Agrobiological Sciences
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- Kusaba Makoto
- Graduate School of Agricultural and Life Sciences, University of Tokyo
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- Iida Shuichi
- Chugoku National Agricultural Research Center for Western region, National Agriculture and Bio-oriented Research Organization
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- Nishio Takeshi
- Graduate School of Agricultural Sciences, Tohoku University
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- Nishimura Minoru
- Institute of Radiation Breeding, National Institute of Agrobiological Sciences
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抄録
In the course of evolution, a gene is often duplicated in tandem, resulting in a functional redundancy. The analysis of function of these genes by raising double mutant might be difficult because they are very tightly linked. We described here a mutant of such a tandem duplicated gene. glu1 is a gamma-ray-induced rice mutant, which lacks an acidic subunit of glutelin, a major seed storage protein. We found that glu1 harbors a 129.7-kb deletion involving two highly similar and tandem repeated glutelin genes, GluB5 and GluB4. The deletion eliminated the entire GluB5 and GluB4 gene except half of the first exon of GluB5. GluB5 and GluB4 have the same amino acid sequence in the acidic subunit, suggesting that only the mutation involving both GluB5 and GluB4 results in the lack of the glutelin acidic subunit deleted in glu1. Our finding suggests that gamma-ray can be an effective mutagen to analyze tandem repeated and functionally redundant genes.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 82 (4), 321-327, 2007
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詳細情報 詳細情報について
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- CRID
- 1390001205474644864
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- NII論文ID
- 10024394759
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 9304387
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 使用不可