Expression of Hedgehog Family Genes in the Rat Uterus During Early Pregnancy

Access this Article

Author(s)

    • WATANABE Ryo
    • Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
    • OOZONO Shinji
    • Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
    • ARAMAKI Shinya
    • Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
    • WOOD Chris
    • Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University
    • SOH Tomoki
    • Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
    • HATTORI Masa-Aki
    • Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University

Abstract

Hedgehog (Hh) plays a pivotal role in various tissues during embryonic development, tissue homeostasis and tumorigenesis. In mammals, Hh exists in three homologs: Desert hedgehog (<i>Dhh</i>), Indian hedgehog (<i>Ihh</i>) and Sonic hedgehog (<i>Shh</i>). In this study, we cloned full-length cDNAs encoding Dhh and Ihh from the rat uterus. Their amino acid sequences have a high homology with those of the mouse and human. In addition, the changes of Hh gene expression in the rat uterus during early pregnancy were analyzed. The results showed that all three hedgehog mRNAs were detected in the rat uterus at the proestrus stage and during early pregnancy (1.5, 3.5, 5.5 and 7.5 days post coitus: dpc). <i>Ihh</i> mRNA expression varied and peaked at 3.5 dpc in the luminal and glandular epithelium. Expression was decreased on 5.5 dpc with the exception of sustained expression in the glandular epithelium. Despite such <i>Ihh</i> variability, the expressions of <i>Dhh</i> and <i>Shh</i> mRNA remained unchanged. This indicated that <i>Ihh</i> was mainly expressed in the rat uterus during early pregnancy. Moreover, the Hh target gene (glioma-associated oncogene homolog 1; <i>Gli</i>1) was also highly expressed at 3.5 dpc in the epithelium and periepithelial stroma in a manner similar to the temporal pattern of <i>Ihh</i> expression. This suggests that <i>Ihh</i> signaling axis play a role in the rat uterus during early pregnancy. In summary, our results elucidate that <i>Ihh</i> is a predominant Hh protein in the rat uterus during early pregnancy and that other Hhs have the potential to be expressed. This observation will help to elucidate the basic molecular mechanism of rat uterus during early pregnancy.<br>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 54(5), 340-345, 2008-10-01

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

References:  28

Codes

  • NII Article ID (NAID)
    10024412256
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168818
  • NDL Article ID
    9690019
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z54-H305
  • Data Source
    CJP  NDL  J-STAGE 
Page Top