Study of the Potentiality of a Cell Culture Medium of Autologous Blood Serum for Cell Transplantation Therapy for Injured Vocal Folds

• Kanemaru Shin-ichi

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Umeda Hiroo

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Suhiro Atsushi

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Ohono Tsunehisa

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Kitani Yoshiharu

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Kishimoto Yo

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Tamura Yoshihiro

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Hirano Shigeru

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

• Omori Koichi

  Department of Otolaryngology Fukushima Medical University School of Medicine

• Ito Juichi

  Department of Otolaryngology- Head and Neck Surgery, Graduate School of Medicine, Kyoto University

Bibliographic Information

Other Title

• 細胞移植による声帯再生のための細胞培養液の検討

Abstract

We succeeded in the regeneration of an injured vocal fold in a canine by the transplantation of bone marrow derived stromal cells (BSCs). This therapy required a cell culture. The safety of the ordinal culture medium with an animal serum such as fetal bovine serum (FBS), however, has not yet been confirmed. In this in vitro study, we investigated the potentiality of a culture medium of autologous blood serum (ABS) which would be the safest additive for clinical applications.<BR>Canine ABS and the following three types of medium were prepared. Mediums I, II and III were alpha-Minimum Essential Medium (alpha-MEM) as blood serum free medium for control, alpha-MEM with 15% ABS and Dulbecco's Modified Eagle's Medium (D-MEM) with 10% FBS for ordinal medium, respectively. The growth rates of the canine cultured BSCs in these three mediums were estimated.<BR>After 2 weeks culture, the number of cells in medium I, II and III increased by 2, 8-10 and 14-15 times, respectively. These results showed that ABS had a satisfactory cell growth promoting action to apply clinically as a substitute for FBS.

Journal

• Koutou (THE LARYNX JAPAN)

  Koutou (THE LARYNX JAPAN) 20 (1), 1-4, 2008

  The Japan Laryngological Association

Keywords

• regeneration of the injured vocal fold
• bone marrow derived stromal cells
• culture medium
• fetal bovine serum (FBS)
• autologous blood serum (ABS)

Details

• CRID: 1390001205463618816
• NII Article ID: 10024456971
• NII Article ID: 130004647250
• NII Book ID: AN10200295
• DOI: 10.5426/larynx1989.20.1_1
• ISSN: 09156127
• Web Site: https://search.jamas.or.jp/link/ui/2008299296
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed