黒毛和種未経産牛におけるCIDRとFSHを用いた過剰排卵処理成績に及ぼすEstradiol-17βの効果

DOI Web Site 被引用文献4件 参考文献26件

書誌事項

タイトル別名
  • Effect of Estradiol-17β on Superovulatory Treatment with CIDR and FSH in Japanese Black Heifers
  • クロゲワシュ ミ ケイサン ウシ ニ オケル CIDR ト FSH オ モチイ

この論文をさがす

抄録

The aims of the present study were to examine the effect of injection of estradiol-17βvalerate (EV) on superovulatory treatment with CIDR-B (CIDR) and FSH in Japanese Black heifers and the effect of repeated superovulatory treatments at a short time interval. Sixteen heifers were allocated ramdomly to the following two treatments, treatment 1 and treatment 2. Each heifer received CIDR in the vagina regardless the day of estrus cycle. On the following day heifers allocated to treatment 1 were injected intramuscularly with EV 5mg dissolved in 2ml of sesame oil. Similarly heifers allocated to treatment 2 were injected intramuscularly with 2ml of sesame oil as a vehicle. Treatment 2 served as control. Superovulatory treatment was started on day 5 after the EV/sesame oil injection. Briefly, heifers received intramuscularly 20 AU FSH in a decreasing manner for 3 days. On day 3 after the first injection of FSH, CIDR was removed from each heifer and simultaneously 750μg cloprostenol was injected intramuscularly. When heifers showed estrus, the cows were inseminated artificially with frozen semen twice at an interval of 12h. Embryo collection was carried out nonsurgically on day 7 after showing standing estrus. A combination of the superovulatory treatment and the embryo collection was one round. In total, four rounds were performed reversing treatments at each round. An interval of embryo collection was uniformly 28 days. At 3rd and 4th rounds the number of donor heifers were 12 as a result of removal of 4 donors. At 1st and 2nd rounds follicular dynamics of ovaries were observed every two days from the day of CIDR insertion to the day of CIDR removal with an ultrasonography. As a result of an analysis of variance with data from 12 heifers during 4 rounds, the number of ova recovered was increased significantly by EV injection (P<0.05). The number 13 of corpora lutea per donor was affected significantly by the number of times of superovulatory treatment (P<0.05). As a result of every continuous two rounds, embryo collection in treatment 1 was significantly higher than that in treatment 2 when the number of ova recovered in treatment 1 was less than 10 or 8. In contrast, when the number of ova recovered in treatment 1 was more than 10 or 8, no significant difference was observed between treatments 1 and 2. From the result of ultrasonographic observation for ovaries, it was evident that the number of large follicles (more than 8mm in diameter) at the initial day of superovulatory treatment was significantly lower in treatment 1 than treatment 2. The result of the present study suggests that the superovulatory treatment with CIDR and EV could control the number of large follicles and improve superovulatory response.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 68 (11), 1075-1084, 1997

    公益社団法人 日本畜産学会

被引用文献 (4)*注記

もっと見る

参考文献 (26)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ