最新のキャピラリー電気泳動とチップ電気泳動　　ＣＥ‐ＭＳによるメタボローム解析

• 曽我 朋義

  慶應義塾大学先端生命科学研究所

書誌事項

タイトル別名

• Metabolome analysis by CE-MS.
• CE-MSによるメタボローム解析
• CE MS ニ ヨル メタボローム カイセキ

抄録

Metabolomics, which can be defined as the measurement of the level of all intracellular metabolites, has become a powerful new tool for gaining insight into cellular and physiological responses. Here we have developed a comprehensive and quantitative analysis method based on capillary electrophoresis mass spectrometry (CE-MS). In this marriage of techniques, CE confers rapid analysis and efficient resolution, and MS provides excellent selectivity and sensitivity. Previous work in our laboratory demonstrated that CE-MS techniques were quite useful for the metabolome analysis. We applied this approach to profile liver metabolites following acetaminophen-induced hepatotoxicity and revealed ophthalmate as a sensitive indicator of reduced glutathione (GSH) depletion. Our results specifically indicate that serum ophthalmate is a sensitive indicator of hepatic GSH depletion, and may be a new biomarker for oxidative stress.<br>

収録刊行物

• 生物物理化学

  生物物理化学 52 (3), 91-94, 2008

  日本電気泳動学会

キーワード

• metabolomics
• capillary electrophoresis mass spectrometry
• drug induced hepatotoxicity
• biomarker
• glutathione

詳細情報

• CRID: 1390001204201054336
• NII論文ID: 10024589091
• NII論文ID: 130004456474
• NII書誌ID: AN00129729
• DOI: 10.2198/sbk.52.91
• COI: 1:CAS:528:DC%2BD1cXhtlansb7M
• ISSN: 13499785
• ISSN: 00319082
• NDL書誌ID: 9687275
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9687275
• Web Site: https://search.jamas.or.jp/link/ui/2009005448
• 本文言語コード: ja
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可