Gene disruption analysis of two glycosylation steps in lankamycin biosynthesis in Streptomyces rochei

• Arakawa Kenji

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

• Suzuki Toshihiro

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

• Kinashi Haruyasu

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

抄録

Lankamycin, produced by Streptomyces rochei 7434AN4, is a 14-membered macrolide antibiotic attached with two deoxysugars, 4″-O-acetyl-L-arcanose and D-chalcose. To reveal the order of glycosylation steps in lankamycin biosynthesis, we carried out gene disruption of two glycosyltransferase genes, lkmI and lkmL. The lkmI mutant KA50 produced 3-O-L-arcanosyl lankanolide, while the lkmL mutant KA55 accumulated two aglycons, 8-deoxylankanolide and 8,15-dideoxy-15-oxolankanolide. These results indicated that LkmL transfers L-arcanose to the C-3 hydroxyl of 8-deoxylankanolide, while LkmI does D-chalcose to the C-5 hydroxyl of 3-O-L-arcanosyl lankanolide. Taking together with previous results of gene disruption of two P450 hydroxylases, we propose a biosynthetic pathway of lankamycin including two hydroxylation and two glycosylation steps.

収録刊行物

• 日本放線菌学会誌

  日本放線菌学会誌 22 (2), 35-41, 2008

  日本放線菌学会

キーワード

• General Medicine

詳細情報

• CRID: 1390001204702751360
• NII論文ID: 10024656843
• NII論文ID: 130004487568
• NII書誌ID: AN10080091
• DOI: 10.3209/saj.saj220202
• ISSN: 18816371
• ISSN: 09145818
• NDL書誌ID: 9781058
• Web Site: http://id.ndl.go.jp/bib/9781058
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9781058
• 本文言語コード: en
• データソース種別:
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