Improvement of Biocompatibility of Chitosan Fiber Modified by Ca-Phosphate Deposition through an Alternate Soaking Process
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- Yoshioka Tomohiko
- Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology
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- Onomoto Hiroshi
- Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology
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- Kashiwazaki Haruhiko
- Department of Gerodontology, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University
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- Inoue Nobuo
- Department of Gerodontology, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University
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- Koyama Yoshihisa
- Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
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- Takakuda Kazuo
- Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
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- Tanaka Junzo
- Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology
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Abstract
A tube consisted of chitin/chitosan nanofibers is one of the candidates to support nerve regeneration. However, chitin/chitosan would cause inflammation when implanted in human body. This study indicates that chitosan nanofibers (degree of deacetylation; 78% and 93%) could be modified by alternate soaking in calcium and phosphate solutions, resulting in the improvement of their biocompatibility in terms of both in vitro cell culture experiment and in vivo animal test. Deacetylation of the chitosan nanofibers appeared to have little effect on in vitro and in vivo behaviors after 3 days. However, the alternate soaking process clearly improved in vitro and in vivo responses against the chitosan nanofibers. During the process, the surface morphology of the chitosan nanofiber was not changed, i.e. no hydroxyapatite was deposited due to the limited number of soaking. Thus, the surface chemical and physical states of the modified chitosan nanofiber such as surface charge, surface free-energy, and wettability was found to influence both the cell attachment and inflammation.
Journal
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- MATERIALS TRANSACTIONS
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MATERIALS TRANSACTIONS 50 (6), 1269-1272, 2009
The Japan Institute of Metals and Materials
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Keywords
Details 詳細情報について
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- CRID
- 1390001204250240640
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- NII Article ID
- 10024815520
- 130004454274
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- NII Book ID
- AA1151294X
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- ISSN
- 13475320
- 13459678
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- NDL BIB ID
- 10236875
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed