T Cell Receptor Repertoire in BALB/c Mice Varies According to Tissue Type, Sex, Age, and Hydrocortisone Treatment

• KITAURA Kazutaka

  Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital Department of Virology I, National Institute of Infectious Diseases Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba

• KANAYAMA Kiichi

  Laboratory of Veterinary Physiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

• FUJII Yoshiki

  Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital Department of Virology I, National Institute of Infectious Diseases

• SHIOBARA Noriyuki

  Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital

• TANAKA Konagi

  Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital

• KURANE Ichiro

  Department of Virology I, National Institute of Infectious Diseases

• SUZUKI Satsuki

  Section of Biological Science Research Center for Odontology, Nippon Dental University School of Dentistry at Tokyo

• ITOH Tsunetoshi

  Division of Immunology and Embryology, Department of Cell Biology, Tohoku University School of Medicine

• SUZUKI Ryuji

  Department of Rheumatology and Clinical Immunology, Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital

抄録

Diversity in T cell recognition of antigens is determined by diverse usage of T cell receptor (TCR) repertoire. TCR repertoire analysis provides fundamental information for understanding T cell immune responses in the pathogenesis of various diseases. In the present study, we examined the TCR repertoire in various tissues in normal BALB/c mice. The TCR α chain variable region repertoires were consistent among the spleen, lymph nodes, and the thymus. The TCR β chain variable region (TCRBV) repertoires were consistent between the spleen and lymph nodes, but different in the thymus. The TCR repertoires also differed in the lungs and the intestinal tract. The TCR repertoires were consistent between male and female mice, except for TCRBV15-1. TCR repertoire was almost similar in 3- and 7-week-old mice, except for TCRBV1-1, 8-3, and 14-1. The present findings suggest that the TCR repertoire of mice varies according to tissue type, sex and age. Additional analysis of the TCR repertoire, i.e., the effect of hydrocortisone (HC), was carried out. After the HC treatment, although the thymic T cells decreased to one-tenth, only a small fraction of CD4⁺CD8⁺ T cells survived the treatment. Furthermore, the percentages of thymic T cells bearing TCRBV3-1, 5-1, 5-2, and 16-1 substantially decreased, but the percentage of cells bearing TCRBV12-1 did not decrease. The present findings suggest that the HC susceptibility of immature thymic T cells is different between TCR families.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 58 (2), 159-168, 2009

  公益社団法人 日本実験動物学会

キーワード

• FACS
• hydrocortisone
• real-time PCR
• repertoire
• T cell receptor

詳細情報

• CRID: 1390001205045002112
• NII論文ID: 130000122130
• NII論文ID: 10024838349
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.58.159
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 10206596
• Web Site: http://id.ndl.go.jp/bib/10206596
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10206596
• Web Site: http://www.jstage.jst.go.jp/article/expanim/58/2/58_2_159/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009252209
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
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