High-throughput determination of thioglycolic acid lignin from rice

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Author(s)

Abstract

For research and development of plants suitable for forage and biofuel production, the development of a high-throughput system of lignin determination, especially for herbaceous species, is important. However, currently available methods for lignin determination are not suitable for high-throughput analysis of herbaceous samples. In this paper, we describe a straightforward, high-throughput method for lignin determination as thioglycolic acid lignin from rice straw using disposable plastic microtubes, TissueLyser, and a UV microplate reader. We successfully plotted calibration curves derived from the readings of a spectrophotometer and a microplate reader using thioglycolic acid lignin prepared from bamboo milled wood lignin as a lignin standard. Based on the calibration curve, we could determine the lignin content in various organs from dried rice straw and rice seedlings. We confirmed that 20 mg of dried material from rice plants is adequate for stable determination of the lignin content. Using this method, 100 independent samples from rice straw can be analyzed in three days per person.

Journal

  • Plant Biotechnology

    Plant Biotechnology 26(3), 337-340, 2009-06-01

    Japanese Society for Plant Cell and Molecular Biology

References:  14

Cited by:  9

Codes

  • NII Article ID (NAID)
    10024853979
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424580
  • NDL Article ID
    10258809
  • NDL Source Classification
    ZR3(科学技術--生物学--植物)
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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