High-throughput screening for plant defense activators using a β-glucuronidase-reporter gene assay in Arabidopsis thaliana
-
- Narusaka Yoshihiro
- Research Institute for Biological Sciences (RIBS) Okayama
-
- Narusaka Mari
- Research Institute for Biological Sciences (RIBS) Okayama Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
-
- Abe Hiroshi
- Experimental Plant Division, RIKEN Bio Resource Center
-
- Hosaka Nami
- Research Institute for Biological Sciences (RIBS) Okayama
-
- Kobayashi Masatomo
- Experimental Plant Division, RIKEN Bio Resource Center
-
- Shiraishi Tomonori
- Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
-
- Iwabuchi Masaki
- Research Institute for Biological Sciences (RIBS) Okayama
書誌事項
- タイトル別名
-
- High-throughput screening for plant defense activators using a .BETA.-glucuronidase-reporter gene assay in Arabidopsis thaliana
- High throughput screening for plant defense activators using a v glucuronidase reporter gene assay in Arabidopsis thaliana
- High-throughput screening for plant defense activators using a β-glucuronidase-reporter gene assay in <italic>Arabidopsis thaliana</italic>
この論文をさがす
抄録
To develop a screening system for plant defense activators, which are novel substances that protect plants by enhancing their inherent disease-resistance mechanisms, we utilized a GUS histochemical staining assay using promoters of the defense-related genes, PR-1 and PDF1.2. We can perform about 1,000 screenings per week per person by this high-throughput screening method. This GUS assay for plant defense activator candidates was evaluated by QRT-PCR analysis to elucidate the functions of the plant defense activators in detail. In the present preliminary screening, we evaluated two hundred chemicals chosen at random. Some chemicals induced GUS activity in a PR-1 promoter::GUS transformant, i.e., abietic acid, allose, glycine, and thymol. The induction of PR-1 expression by the treatments with these chemicals was confirmed using QRT-PCR. The foliar treatment with abietic acid 1 d prior to inoculation with the fungal pathogen Colletotrichum higginsianum led to a significant reduction of necrotic surface area compared with distilled water treated controls, as observed 6 d after inoculation. These results suggest that this GUS histochemical staining assay is an effective and available screening system for plant defense activators.
収録刊行物
-
- Plant Biotechnology
-
Plant Biotechnology 26 (3), 345-349, 2009
日本植物バイオテクノロジー学会
- Tweet
キーワード
詳細情報
-
- CRID
- 1390282679303710976
-
- NII論文ID
- 10024854029
-
- NII書誌ID
- AA11250821
-
- ISSN
- 13476114
- 13424580
-
- NDL書誌ID
- 10258868
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可