Effects of Target Sequence and Sense versus Anti-sense Strands on Gene Correction with Single-stranded DNA Fragments

• Kamiya, Hiroyuki

• Uchiyama, Masayuki

• Nakatsu, Yoshimichi

• Tsuzuki, Teruhisa

• Harashima, Hideyoshi

抄録

The correction of an inactivated hygromycin resistance and enhanced green fluorescent protein (Hyg–EGFP) fusion gene by a several hundred-base single-stranded (ss) DNA fragment has been reported. In this study, the effectiveness of this type of gene correction was examined for various positions in the rpsL gene. Sense and anti-sense ssDNA fragments were prepared, and the gene correction efficiencies were determined by co-introduction of the target plasmid containing the gene with the ssDNA fragments. The gene correction efficiency varied (0.8–9.3%), depending on target positions and sense/anti-sense strands. Sense ssDNA fragments corrected the target gene with equal or higher efficiencies as compared to their anti-sense counterparts. The target positions corrected with high efficiency by the sense fragments also tended to be corrected efficiently by the anti-sense fragments. These results suggest that the sense ssDNA fragments are useful for the correction of mutated genes. The variation in the correction efficiency may depend on the sequence of the target position in double-stranded DNA.

収録刊行物

• Journal of Biochemistry

  Journal of Biochemistry 144 (4), 431-436, 2008-10

  The Japanese Biochemical Society

キーワード

• gene correction
• genetic engineering
• nucleic acid therapeutics
• rpsL gene
• single-stranded DNA fragment

詳細情報

• CRID: 1050564288954301696
• NII論文ID: 10024912605
• NII書誌ID: AA00694073
• ISSN: 17562651
• ISSN: 0021924X
• HANDLE: 2115/38723
• NDL書誌ID: 9688189
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9688189
• Web Site: https://search.jamas.or.jp/link/ui/2009106594
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • NDL
  • CiNii Articles