Validation in diastolic function (E/A) in rats

• ISHIKURA Fuminobu

  The School of Allied Health Sciences,Osaka University,Faculty of Medicine

• ASANUMA Toshihiko

  The School of Allied Health Sciences,Osaka University,Faculty of Medicine

• YAMAMOTO Kazuhiro

  The Center for Advanced Medical Engineering and Informatics,Osaka University

• OHMORI Koji

  The Second Department of Internal Medicine,Kagawa University School of Medicine

• TAKANO Masumi

  The First Department of Internal Medicine,Fukushima Medical University

• BEPPU Shintaro

  The School of Allied Health Sciences,Osaka University,Faculty of Medicine

Bibliographic Information

Other Title

• ラットにおける拡張能（Ｅ／Ａ）評価の妥当性

Abstract

Purpose: To evaluate the effect of anesthesia on cardiac function. Mitral inflow pattern, especially E/A ratio, is very useful in evaluating diastolic function; however, it is not easy to measure E/A in small animals like mice and rats because of the summations induced by their high heart rate. E/A can generally be measured by administering an anesthetic agent to reduce heart rate, although the effect of anesthesia on cardiac function is unclear. Methods: Sprague Dawley rats were studied by ViVid 7 (GE Healthcare, USA) with a high-frequency transducer (11.5 MHz, 234 FPS) to evaluate fractional area changes (FAC) and mitral flow pattern after step-wise increase in anesthesia. Results: The A wave gradually separated from the E wave as heart rate decreased, and E/A could be measured in all the rats when heart rate was reduced to 300 bpm. Change in fractional area decreased slightly, from 71.4 ± 4.6% to 67.6 ± 3.9%, with reduction in heart rate. Conclusion: The E/A ratio in rats could be measured after heart-rate reduction with little decrease in fractional area changes. The measure of E/A after heart rate reduction may be acceptable except in the special case of evaluating microscopic changes.

Journal

• Choonpa Igaku

  Choonpa Igaku 35 (1), 13-18, 2008

  The Japan Society of Ultrasonics in Medicine

Keywords

• diastolic function
• E/A
• echocardiography
• left ventricular systolic function
• small animals

Details

• CRID: 1390282680175073024
• NII Article ID: 10024927418
• NII Book ID: AA11889584
• DOI: 10.3179/jjmu.35.13
• ISSN: 18819311
• ISSN: 13461176
• Web Site: https://search.jamas.or.jp/link/ui/2008120823
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed