Nuclear Transfer Preserves the Nuclear Genome of Freeze-Dried Mouse Cells
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- ONO Tetsuo
- Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology Department of Medical Science, Graduate School of Medicine, Kyoto University
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- MIZUTANI Eiji
- Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology
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- LI Chong
- Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology Department of Bioscience, Graduate School of Science and Technology, Kwansei Gakuin University
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- WAKAYAMA Teruhiko
- Laboratory for Genomic Reprogramming, RIKEN Center for Developmental Biology Department of Medical Science, Graduate School of Medicine, Kyoto University Department of Bioscience, Graduate School of Science and Technology, Kwansei Gakuin University
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Mouse spermatozoa can be freeze dried without losing genetic integrity and reproductive potential. However, it is not known if freeze-dried mouse cells similarly maintain their genetic integrity and developmental potential following nuclear transfer. Here, we investigated the developmental capacity and embryonic stem (ES) cell derivation of reconstructed oocytes by nuclear transfer using freeze-dried cumulus or ES cells. Cumulus and ES cells were lyophilized overnight and stored at 4 C for up to 1 week. After rehydration, all cells showed membrane damage and were unviable. However, following nuclear transfer, 1-4% of the reconstructed oocytes developed to the blastocyst stage. A total of five nuclear transfer ES (ntES) cell lines were generated from blastocysts and morulae. All ntES cell lines had normal karyotypes and were positive for the ES-cell-specific markers (alkaline phosphatase, Oct3/4 and Nanog). After aggregation of ntES cells with fertilized embryos, chimeric mice with a high level of coat color chimerism were generated. Our findings show that the genomic integrity of cells can be maintained after freeze-drying and that it is possible to produce offspring from the cells using nuclear transfer techniques.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 54 (6), 486-491, 2008
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337515648
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- NII論文ID
- 10024940504
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 9752794
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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