Chicken Dead End Homologue Protein is a Nucleoprotein of Germ Cells Including Primordial Germ Cells

DOI Web Site Web Site PubMed 被引用文献7件 参考文献40件
  • ARAMAKI Shinya
    Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
  • KUBOTA Kaiyu
    Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
  • SOH Tomoki
    Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
  • YAMAUCHI Nobuhiko
    Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University
  • HATTORI Masa-aki
    Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University

この論文をさがす

抄録

The dead end gene, coding an RNA binding protein, is predominantly expressed in the germ cells of vertebrates. Recently, we cloned chicken dead end homologue (CDH) and showed that expression of CDH mRNA is highly specific to primordial germ cells (PGCs) at early embryonic stages. To date, the subcelluler localization of Dead end protein in germ cell has been largely unknown due to lack of an antibody. Here, we raised a polyclonal antibody against chicken dead end homologue (CDH) to elucidate its subcellular localization in the germ cells. For comparative studies with CDH, a polyclonal antibody against chicken vasa homologue (CVH), a well-known germ cell marker, was also raised. Immunoblotting analysis for CDH protein showed a single band with a molecular size of approximately 60 kDa in the ovarian and testicular proteins. Immunofluorescence studies revealed that CDH protein was exclusively localized in the nuclei of primordial germ cells (PGCs) and germ cells at later stages, while CVH was localized in the cytoplasm. Interestingly, the germ cells distributed at the basal sides of seminiferous epithelia, such as spermatogonia, were strongly positive to CDH protein. The current study provides novel evidence that CDH is a nucleoprotein of germ cells, including PGCs.<br>

収録刊行物

被引用文献 (7)*注記

もっと見る

参考文献 (40)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ