Microtubule remodeling mediates the inhibition of store-operated calcium entry (SOCE) during mitosis in COS-7 cells

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  • Russa Afadhali Denis
    Department of Anatomy (Cell Biology Group), Iwate Medical University School of Medicine Department of Anatomy and Histology, Muhimbili University of Health and Allied Sciences, School of Medicine
  • Ishikita Naoyuki
    Department of Anatomy (Cell Biology Group), Iwate Medical University School of Medicine
  • Masu Kazuki
    Department of Anatomy (Cell Biology Group), Iwate Medical University School of Medicine
  • Akutsu Hitomi
    Department of Anatomy (Cell Biology Group), Iwate Medical University School of Medicine Advanced Medical Science Center, Iwate Medical University School of Medicine
  • Saino Tomoyuki
    Department of Anatomy (Cell Biology Group), Iwate Medical University School of Medicine
  • Satoh Yoh-ichi
    Department of Anatomy (Cell Biology Group), Iwate Medical University School of Medicine

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Abstract

Regulation of the intracellular calcium ion concentration ([Ca2+]i) is critical, because calcium signaling controls diverse and vital cellular processes such as secretion, proliferation, division, gene transcription, and apoptosis. Store-operated calcium entry (SOCE) is the main mechanism through which non-excitable cells replenish and thus maintain this delicate balance. There is limited evidence which indicates that SOCE may be inhibited during mitosis, and the mechanisms leading to the presumed inhibition has not been elucidated. In the present study, we examined and compared the [Ca2+]i dynamics of COS-7 cells in mitotic and non-mitotic phases with special reference paid to SOCE. Laser scanning confocal microscopy to monitor [Ca2+]i dynamics revealed that SOCE was progressively inhibited in mitosis and became virtually absent during the metaphase. We used various cytoskeletal modifying drugs and immunofluorescence to assess the contribution of microtubule and actin filaments in SOCE signaling. Nocodazole treatment caused microtubule reorganization and retraction from the cell periphery that mimicked the natural mitotic microtubule remodeling that was also accompanied by SOCE inhibition. Short exposure to paclitaxel, a microtubule-stabilizing drug, bolstered SOCE, whereas long exposure resulted in microtubule disruption and SOCE inhibition. Actin-modifying drugs did not affect SOCE. These findings indicate that mitotic microtubule remodeling plays a significant role in the inhibition of SOCE during mitosis.

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