Development of Semi-Separated Co-Culture System for Electrical Stimulation and Extracellular Recording of Sympathetic Neuron and Cardiomyocyte
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- Takeuchi Akimasa
- Graduate School of Frontire Sciences, University of Tokyo
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- Moriguchi Hiroyuki
- Graduate School of Frontire Sciences, University of Tokyo
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- Kotani Kiyoshi
- Graduate School of Frontire Sciences, University of Tokyo
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- Lee Jong-kook
- Research Institute of Environmental Medicine, Nagoya University
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- Noshiro Makoto
- Graduate School of Medical Sciences, Kitasato University
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- Jimbo Yasuhiko
- Graduate School of Frontire Sciences, University of Tokyo
Bibliographic Information
- Other Title
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- 電気刺激/計測可能な交感神経と心筋細胞の半独立共培養系の構築
- デンキ シゲキ ケイソク カノウ ナ コウカン シンケイ ト シンキン サイボウ ノ ハンドクリツ キョウ バイヨウケイ ノ コウチク
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Abstract
A semi-separated co-culture system for spatio-temporal recording of the electrical activities from superior cervical ganglion (SCG) neurons and ventricular myocytes (VMs) was developed by using a handmade “H-shaped” chamber placed on microelectrode arrays (MEA). The chamber was made of polydimethylsyloxane (PDMS) and consisted of two chambers which were connected with a pathway. 16-20 hours after dissemination of SCG neurons into one chamber, the dissociated VMs were disseminated into the other chamber. 4days after dissemination of VMs, SCG neurons and VMs conjugated only at the pathway. Spontaneous electrical activities of SCG neurons and VMs were observed several days after the dissemination of VMs. Constant-voltage stimualtion (1 V, 1 ms, biphasic square pulses) was applied to SCG neurons at the frequency of 10 Hz using 32 electrodes. After applying electrical stimulation to SCG neurons, the contraction rate of VMs in three samples increased by 55±5.6%, 64±8.8%, 280±160%, respectively. This result suggests that neuromuscular junctions were formed between SCG neurons and VMs.
Journal
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- IEEJ Transactions on Electronics, Information and Systems
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IEEJ Transactions on Electronics, Information and Systems 129 (7), 1225-1230, 2009
The Institute of Electrical Engineers of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679581311104
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- NII Article ID
- 10025101111
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- NII Book ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL BIB ID
- 10357161
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed