Signal Enhancement for Gene Detection Based on a Redox Reaction of [Fe(CN)6]4- Mediated by Ferrocene at the Terminal of a Peptide Nucleic Acid as a Probe with Hybridization-amenable Conformational Flexibility

• AOKI Hiroshi

  National Institute of Advanced Industrial Science and Technology (AIST)

• TAO Hiroaki

  National Institute of Advanced Industrial Science and Technology (AIST)

Bibliographic Information

Other Title

• Signal enhancement for gene detection based on a redox reaction of 〔Fe(CN)6〕[4-] mediated by ferrocene at the terminal of a peptide nucleic acid as a probe with hybridization-amenable conformational flexibility

Abstract

Electrochemically enhanced DNA detection was demonstrated by utilizing the couple of a synthesized ferrocene-terminated peptide nucleic acid (PNA) with a cysteine anchor and a sacrificial electron donor [Fe(CN)₆]^4-. DNA detection sensors were prepared by modifying a gold electrode surface with a mixed monolayer of the probe PNA and 11-hydroxy-1-undecanethiol (11-HUT), protecting [Fe(CN)₆]^4- from any unexpected redox reaction. Before hybridization, the terminal ferrocene moiety of the probe was subject to a redox reaction due to the flexible probe structure and, in the presence of [Fe(CN)₆]^4-, the observed current was amplified based on regeneration of the ferrocene moiety. Hybridization decreased the redox current of the ferrocene. This occurred because hybridization rigidified the probe structure: the ferrocene moiety was then removed from the electrode surface, and the redox reaction of [Fe(CN)₆]^4- was again prevented. The change in the anodic current before and after hybridization was enhanced 1.75-fold by using the electron donor [Fe(CN)₆]^4-. Sequence-specific detection of the complementary target DNA was also demonstrated.

Journal

• Analytical Sciences

  Analytical Sciences 24 (7), 929-933, 2008

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204256412160
• NII Article ID: 130004441685
• NII Article ID: 10025205267
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.24.929
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 9566464
• Web Site: http://id.ndl.go.jp/bib/9566464
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9566464
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.24.929.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.24.929/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/24/7/24_7_929/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed