Bioluminescent Indicators for Ca2+ Based on Split Renilla Luciferase Complementation in Living Cells
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- KAIHARA Asami
- Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University Department of Chemistry, Graduate School of Science, University of Tokyo
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- UMEZAWA Yoshio
- Department of Chemistry, Graduate School of Science, University of Tokyo Research Institute of Pharmaceutical Sciences, Musashino University
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- FURUKAWA Tetsushi
- Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University
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抄録
Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emission of bioluminescence split Renilla luciferase. This is how intracellular Ca2+ is illuminated with the intramolecular complementation of split Renilla luciferase. The Ca2+-dependent spontaneous and simultaneous emission of bioluminescence promises to reveal Ca2+ dynamics in living cells, and also in vivo using the present indicators.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 24 (11), 1405-1408, 2008
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204257032064
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- NII論文ID
- 130004441578
- 10025207918
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BD1cjivVSguw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 9697283
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- PubMed
- 18997366
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I9697283
- https://link.springer.com/content/pdf/10.2116/analsci.24.1405.pdf
- https://link.springer.com/article/10.2116/analsci.24.1405/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/24/11/24_11_1405/_pdf
- https://search.jamas.or.jp/link/ui/2009096676
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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