Bioluminescent Indicators for Ca2+ Based on Split Renilla Luciferase Complementation in Living Cells

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  • KAIHARA Asami
    Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University Department of Chemistry, Graduate School of Science, University of Tokyo
  • UMEZAWA Yoshio
    Department of Chemistry, Graduate School of Science, University of Tokyo Research Institute of Pharmaceutical Sciences, Musashino University
  • FURUKAWA Tetsushi
    Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University

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抄録

Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emission of bioluminescence split Renilla luciferase. This is how intracellular Ca2+ is illuminated with the intramolecular complementation of split Renilla luciferase. The Ca2+-dependent spontaneous and simultaneous emission of bioluminescence promises to reveal Ca2+ dynamics in living cells, and also in vivo using the present indicators.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 24 (11), 1405-1408, 2008

    社団法人 日本分析化学会

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