Live Single-cell Metabolomics of Tryptophan and Histidine Metabolites in a Rat Basophil Leukemia Cell

• MIZUNO Hajime

  Graduate School of Biomedical Sciences, Hiroshima University

• TSUYAMA Naohiro

  Graduate School of Biomedical Sciences, Hiroshima University

• DATE Sachiko

  Graduate School of Biomedical Sciences, Hiroshima University

• HARADA Takanori

  Graduate School of Biomedical Sciences, Hiroshima University

• MASUJIMA Tsutomu

  Graduate School of Biomedical Sciences, Hiroshima University

抄録

A direct and rapid metabolic analysis of a live single cell was performed by live single-cell video-mass spectrometry. The contents of the cytoplasm and a granule were sucked into a nano-electrospray ionization (nano-ESI) tip, and were directly introduced into a Q-TOF mass spectrometer by nano-spray after the addition of an ionization solvent. The metabolic pathways and the locations of tryptophan and histidine metabolites were traced by this method for a cultured rat basophil leukemia cell line (RBL-2H3). The t-values of detected peaks by a t-test between the different location, e.g. cytoplasm and a granule, revealed the molecular localization of each MS peak. A direct and quick metabolomic analysis of a living cell under simultaneous video-microscopic observations was innovated.

収録刊行物

• Analytical Sciences

  Analytical Sciences 24 (12), 1525-1527, 2008

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390282679234511616
• NII論文ID: 10025208514
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.24.1525
• COI: 1:STN:280:DC%2BD1M%2FivVSktw%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 9730499
• PubMed: 19075459
• Web Site: http://id.ndl.go.jp/bib/9730499
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9730499
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.24.1525.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.24.1525/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/24/12/24_12_1525/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009144888
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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