Highly Selective and Simple Method for Determination of Polythiols Based on Liquid Chromatography with Postcolumn Excimer Fluorescence Derivatization

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  • YOSHIDA Hideyuki
    Faculty of Pharmaceutical Sciences, Fukuoka University Physical Activity Institute, Fukuoka University
  • SUDO Maki
    Faculty of Pharmaceutical Sciences, Fukuoka University
  • TODOROKI Kenichiro
    Faculty of Pharmaceutical Sciences, Fukuoka University
  • NOHTA Hitoshi
    Faculty of Pharmaceutical Sciences, Fukuoka University
  • YAMAGUCHI Masatoshi
    Faculty of Pharmaceutical Sciences, Fukuoka University Physical Activity Institute, Fukuoka University

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Abstract

An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440 − 520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves.

Journal

  • Analytical Sciences

    Analytical Sciences 25 (6), 829-832, 2009

    The Japan Society for Analytical Chemistry

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