Determination of Short-chain Fatty Acids in Rat and Human Feces by High-Performance Liquid Chromatography with Electrochemical Detection

• KOTANI Akira

  Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• MIYAGUCHI Yuji

  Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• KOHAMA Mototaka

  Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• OHTSUKA Takafumi

  Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

• SHIRATORI Taisei

  Department of Gastroenterology, Hachioji Medical Center of Tokyo Medical University

• KUSU Fumiyo

  Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences

Abstract

A simple method for determining short-chain fatty acids (SCFAs) in rat and human feces was developed using high-performance liquid chromatography with electrochemical detection (HPLC-ECD). A two-channel HPLC-ECD system was fabricated using an ion exclusion column and an electrochemical detector with a glassy carbon working electrode. Aqueous solutions of 0.1 mM HClO₄ and of ethanol containing 2-methyl-1,4-naphthoquinone served as a mobile phase and a quinone solution, respectively. Peak areas for lactic, acetic, propionic, butyric, isovaleric, and valeric acids at a detection potential of −0.9 V vs. an Ag/AgCl electrode showed a linear relationship with the acid amount in the range 0.1 to 40 nmol. Standard acids at 4 nmol were determined ten times with relative standard deviations (RSD) of less than 2.0%. The analytical results of healthy human feces were measured within 35 min. RSD (n = 5) in all SCFAs were less than 2.7%, and recoveries of SCFAs were more than 92%. The present method was characterized by reproducibility with the simple and rapid procedure without derivatization of analytes, and it has the potential for clinical and biomedical applications.

Journal

• Analytical Sciences

  Analytical Sciences 25 (8), 1007-1011, 2009

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679233616384
• NII Article ID: 10025214039
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.25.1007
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 10304485
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10304485
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.25.1007.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.25.1007/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/25/8/25_8_1007/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010012515
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed