In vitro Reconstruction of Visual Information-Processing System Using Co-Cultured Retina and Superior Colliculus
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- Hirota Shinya
- Graduate School of Frontier Sciences, University of Tokyo
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- Moriguchi Hiroyuki
- Graduate School of Frontier Sciences, University of Tokyo
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- Takayama Yuzo
- Graduate School of Frontier Sciences, University of Tokyo
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- Jimbo Yasuhiko
- Graduate School of Frontier Sciences, University of Tokyo
Bibliographic Information
- Other Title
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- 網膜―上丘組織共培養による視覚情報処理系の in vitro 再構築
- モウマク ジョウ キュウ ソシキ キョウ バイヨウ ニ ヨル シカク ジョウホウ ショリケイ ノ in vitro サイコウチク
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Abstract
Retinotectal projection, together with the well-known retinogeniculocortical pathways, plays important roles in visual information processing. In this study, we try in vitro reconstruction of retina-superior-colliculus (SC) pathways on micro- electrode arrays (MEAs). First, retinal tissue and SC slices were prepared from newborn rats and individually cultured on MEA substrates. Spontaneous electrical activity was recorded in both retina and SC cultures. Continuous firing was observed in cultured retina, where the frequency increased from a few Hz to more than 10 Hz with culture duration. Evoked responses were also recorded from cultured retinal tissue. A single biphasic pulse successfully elicited spike trains. Spontaneous activity of SC was observed in the 6 days in vitro (DIV) cultures. Finally, retina and SC were co-cultured under the conditions established for SC-slice cultures. Within a few days, we could observe neurite outgrowth from both tissue and connections were established morphologically. Spontaneous activity was recorded from both retina and SC areas in 11 DIV cultures. The next step will be spatio-temporal analysis of signal-propagation patterns of spontaneous activity as well as SC responses to retinal stimulation.
Journal
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- IEEJ Transactions on Electronics, Information and Systems
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IEEJ Transactions on Electronics, Information and Systems 129 (10), 1923-1928, 2009
The Institute of Electrical Engineers of Japan
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Details 詳細情報について
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- CRID
- 1390282679582503936
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- NII Article ID
- 10025318771
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- NII Book ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL BIB ID
- 10450214
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed