<i>Wx^α</i>遺伝子を発現した糯種イネ胚乳澱粉の分子構造

• 花城 勲

  鹿児島大学農学部生物資源化学科

• 若山 拓志

  鹿児島大学農学部生物資源化学科

• 長谷川 真理

  新潟大学大学院自然科学研究科

• 樋口 利幸

  鹿児島大学農学部生物資源化学科

• 伊藤 紀美子

  新潟大学大学院自然科学研究科 新潟大学超域研究機構

• 福山 利範

  新潟大学農学部農業生産科学科

• 竹田 靖史

  鹿児島大学農学部生物資源化学科

書誌事項

タイトル別名

• Structures of Endosperm Starch from a Rice wx Cultivar Expressing Wx.ALPHA. Transgene
• Structures of endosperm starch from a rice wx cultivar expressing Wx[a] transgene

抄録

Structural changes caused by the introduction of the Wx^α transgene, which encodes granule-bound starch synthase I (GBSSI), into the non-transgenic wx cultivar Musashimochi were examined and compared with the previous results (Plant Cell Physiol., 49, 925-933 (2008), using a transgenic line (WAB3-5) established independently from those used previously. Increases of amylose content of starch and extra-long chain (ELC) content of amylopectin were observed in the line WAB3-5 (actual amylose, 15.2%; ELC, 11.6% by weight). An HPSEC profile (size distribution) of WAB3-5 amylose showed characteristics in-between of those of Wx^α and Wx^b cultivars, and WAB3-5 amylose had a comparably high degree of branching, which was indicated by the molar fraction of branched molecules (MF_B, 0.38) and number of chains of branched molecules (NC_B, 10.5). Amylose from cv. Yumetoiro (Wx^α) had a comparable degree of branching (MF_B, 0.35; NC_B, 13.7) with the WAB3-5 amylose. Chain-length distribution of WAB3-5 amylopectin showed a slightly higher amount of B₂+B₃ chains than cv. Musashimochi. These results are consistent with those of the previous study, providing additional evidence for the proposed role of GBSSI in both amylose and ELC synthesis in rice endosperm. ELCs of amylopectins from line WAB3-5 and cvs. IR36 and Yumetoiro showed size distributions that were basically similar to each other but distinct from those of their amylose counterpart. ELCs were hydrolyzed by β-amylolysis of amylopectins with different extents of trimming, 66.3, 92.0 and 77.0% for line WAB3-5, cv. IR36 and cv. Yumetoiro, respectively, suggesting the branched structure of the ELCs is different among the three amylopectins.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 56 (2), 65-70, 2009

  日本応用糖質科学会

キーワード

• <i>Wx</i>
• granule-bound starch synthase
• amylose content
• extra-long chain

詳細情報

• CRID: 1390282681269031552
• NII論文ID: 10025408877
• NII書誌ID: AN10453916
• DOI: 10.5458/jag.56.65
• COI: 1:CAS:528:DC%2BD1MXhtFeltLzK
• ISSN: 18807291
• ISSN: 13447882
• NDL書誌ID: 10430631
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10430631
• Web Site: http://www.jstage.jst.go.jp/article/jag/56/2/56_2_65/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可