プロテオーム解析における液体クロマトグラフィー3段階質量分析法(LC/MS^3)の有用性について  [in Japanese] Utility of Application of Liquid Chromatography/Triple Stage Mass Spectrometry (LC/MS^3) for Proteome Analysis  [in Japanese]

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Author(s)

Abstract

Protein identification <i>via</i> mass spectrometry (MS) relies primarily on protein sequence databases. Identification resulting from a sequence database search in the tandem mass spectrometry (MS/MS) experiments for the digested peptides is derived from an algorithm based on statistical confidence, and therefore, it often pro- ides false-positive identifications. Although one can apply high-resolution MS such as Fourier-transform mass spectrometer (FT-MS) to obtain results with high confidence, the instrumentation is expensive and the results are still probability based. Therefore, we introduced data-dependent triple stage mass spectrometry (MS<sup>3</sup>) into our well-established liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis of linear ion trap MS to achieve evidence-based protein identification. Peptide sequencing by MS<sup>3</sup> analysis can provide a direct evidence for a sequence elucidated by MS/MS data and help determine whether the search results are correct.

Journal

  • Journal of the Mass Spectrometry Society of Japan

    Journal of the Mass Spectrometry Society of Japan 57(4), 317-325, 2009-08-01

    The Mass Spectrometry Society of Japan

References:  22

Codes

  • NII Article ID (NAID)
    10025571784
  • NII NACSIS-CAT ID (NCID)
    AN0010555X
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    13408097
  • NDL Article ID
    10324162
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z17-213
  • Data Source
    CJP  NDL  J-STAGE 
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