Gold Induces a Metallothionein Gene and a CPx－ATPase Gene Through Regulation of a Repressor BxmR in <i>Oscillatoria brevis</i>

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  • AuストレスはBxmRリプレッサーを介してラン藻<i>Oscillatoria brevis</i>のメタロチオネイン（MT）およびCPx－ATPase遺伝子の発現を制御している
  • Gold induces a metallothionein gene and a CPx-ATPase gene through regulation of a repressor BxmR in Oscillatoria brevis

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Abstract

A cysteine-rich metal binding protein MT (metallothionein) gene (bmtA) and a CPx－ATPase metal transporter gene (bxa1) are induced upon exposure to multiple heavy metal ions in the cyanobacterium Oscillatoria brevis. The SmtB/ArsR family repressor BxmR from O. brevis represses the expression of an operon encoding bmtA and bxmR, and another operon encoding bxa1. In the present study, the expression of bmtA and bxa1 was induced in vivo by 5－15 μM gold (Au) treatment for 1－4 h with a different level in O. brevis cells. In vitro electrophoretic gel mobility shift analysis (EMSA) revealed that the Au treatment promotes a disassembly of BxmR protein from the BxmR－bxmR/bmtA operator (O)/promoter (P) or BxmR－bxa1 O/P complex with a different affinity. Our results indicate that Au treatment can cause a release of BxmR protein in gene-expression level of these genes in O.brevis.

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