Search for Marker Molecules of Diseases by Tandem MS

• Tsuyama Naohiro

  Analytical Molecular Medicine and Devices lab., Graduate School of Biomedical Sciences, Hiroshima University School of Medicine

• Mizuno Hajime

  Analytical Molecular Medicine and Devices lab., Graduate School of Biomedical Sciences, Hiroshima University School of Medicine

• Harada Takanori

  Analytical Molecular Medicine and Devices lab., Graduate School of Biomedical Sciences, Hiroshima University School of Medicine

• Nishigaki Shunta

  Analytical Molecular Medicine and Devices lab., Graduate School of Biomedical Sciences, Hiroshima University School of Medicine

• Masujima Tsutomu

  Analytical Molecular Medicine and Devices lab., Graduate School of Biomedical Sciences, Hiroshima University School of Medicine

• Maeda Masako

  Japan Public Health Association

• Hiyama Eiso

  Natural Science Centre for Basic Research and Development, Hiroshima University

Bibliographic Information

Other Title

• タンデムMSによる疾患関連分子マーカーの探索

Abstract

Neuroblastoma is a tumor of nerve tissue in childhood. Mass-screening program for early detection of neuroblastoma was stopped in Japan because of its inability of precise clarification of malignant tumor. We aimed to find out new tumor markers which could be used in future mass-screening, and have succeeded to establish LC-MS/MS-based quantification of nine urinary catecholamine metabolites. The standard curves of these nine metabolites showed high linearity and reproducibility, thus we applied them to measure the content of the metabolites in patient samples. The results showed a possibility that the ratio of DOPAC and normetanephrine could be a marker of tumors whereas each metabolite alone could not. In addition, we made a survey of candidate neuroblastoma markers in urine using a Q-q-TOF/MS. We found fifteen increased and eight decreased ion peaks in seven tumor samples. We will confirm their ability as diagnostic markers of neuroblastoma by increasing case numbers.

Journal

• Japanese Journal of Clinical Chemistry

  Japanese Journal of Clinical Chemistry 37 (4), 410-417, 2008

  Japan Society of Clinical Chemistry

Keywords

• Neuroblastoma
• Mass spectrometry
• Catecolamines
• Marker survey
• Molecular diagnosis

Details

• CRID: 1390001204910569984
• NII Article ID: 10025632388
• NII Article ID: 130003356903
• NII Book ID: AN00252758
• DOI: 10.14921/jscc1971b.37.4_410
• COI: 1:CAS:528:DC%2BD1cXhtlKhtLrK
• ISSN: 21874077
• ISSN: 03705633
• Web Site: https://search.jamas.or.jp/link/ui/2009039721
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed